Effects of Cu enrichment and release on growth internal quality and relative gene expression of Whitmania pigra
Research article published in Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2018)
Abstract
The present study explored the effects of Cu²⁺enrichment and release on the growth and internal quality of Whitmania pigra, and the regularity of enrichment and release in different tissues of Wh. pigra. In the range of safe concentration(SC), a certain concentration of Cu²⁺ was added to the Wh. pigra for 50 days, and stopped adding for another 50 days. The growth index of Wh. pigra, Cu²⁺ content in different tissues, and the internal quality were determined. The results showed that the average body weight of Wh. pigra in 0.014 mg·L⁻¹ of Cu²⁺ group was significantly higher than that in control group during the experimental period (P<0.05), and the mortality rate was lower than that in the control group. The specific growth rate, digestive enzyme activity, growth and digestive enzyme related gene expression of Wh. pigra in Cu²⁺ group were significantly higher than that in the control group (P<0.05) during the early 40 days of enrichment, and there was no significant difference in the release period. In Cu²⁺ group, the expression of immune enzyme and immune enzyme related gene of Wh. pigra increased first and then decreased compared with control group (P<0.05). The ability of enriched Cu²⁺ in different tissues of Wh. pigra was digestive tract>muscle>skin. The ability of release Cu²⁺ was muscle>digestive tract>skin. There was no significant difference in the internal quality and hirudin gene expression between Cu²⁺ group and control group. In conclusion, Cu²⁺ can improve the expression of digestive enzymes, immune enzyme and related genes of Wh. pigra, promote the growth of Wh. pigra and enhance their immunity, but it doesn't affect the internal quality of Wh. pigra. The Wh. pigra can completely release Cu²⁺ within 30 days.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Zusammenfassung
The present study explored the effects of Cuenrichment and release on the growth and internal quality of Whitmania pigra, and the regularity of enrichment and release in different tissues of Wh.
Warum dies für die Hirudotherapie relevant ist
This study examined the effects of copper (Cu²⁺) enrichment and release on the growth, internal quality, and gene expression of Whitmania pigra over a 100-day period. The relevance to the ASH domain is limited: hirudin gene expression was measured and found unaffected by Cu²⁺ exposure, providing environmental-quality reference data for a leech species. The findings indicate that a safe concentration of Cu²⁺ can promote growth, enhance immunity, and increase digestive enzyme activity without significantly affecting internal quality or hirudin gene expression, with complete Cu²⁺ release within 30 days. However, this study does not address hirudotherapy, the leech secretome, or any clinical application.
Zitation
Effects of Cu enrichment and release on growth internal quality and relative gene expression of Whitmania pigra
Shi GW et al. · Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2018
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