Quantitative Proteomik-basierte Entdeckung bioaktiver Proteine und Qualitätskontrolle medizinischer Blutegel

ETHNOPHARMACOLOGICAL RELEVANCE: Leech, a classical traditional Chinese medicine for promoting blood circulation and removing blood stasis, is mainly used in the clinical treatment of cardiovascular and cerebrovascular diseases. The discovery of activity proteins or peptides in the dead and dried medicinal leech is an important task with great challenges. AIM OF THE STUDY: The aim of this study was to provide a basic proteome profile and help further discover active proteins and quality control for medicinal leeches, which would also provide insight into the research of animal medicines. MATERIALS AND METHODS: Seventeen batches of dried medicinal leeches covering three species were collected from medicinal markets, which were authenticated by DNA barcoding. Then the proteome of different species leeches was profiled to reveal the significantly different proteins using label-free proteomics. The characteristic peptides were screened out based on biological pathways analysis, which were further absolutely quantified using the developed stable isotope-labeled based parallel reaction monitoring method. RESULTS: Seventeen batches of leech materials were Whitmania pigra Whitman (WP), Whitmania laevis Whitman (WL) and Poecilobdella manillensis Lesson (PM), respectively. A total of 1,035 proteins (452 in WP, 425 in WL and 158 in PM) were identified. Among them, 90 overlapping proteins were mainly concentrated in diverse metabolic pathways and primarily localized in the cytoplasm and mitochondrial inner membrane, which mainly related to ATP binding, catalytic activity and structural molecular activity. In total of 51 uniquely expressed proteins (21 in WP, 23 in WL and 7 in PM), associated with multiple key signaling pathways, including Rap1, cGMP-PKG, PI3K-Akt, Wnt and HIF-1, etc., relevant to treating cardiovascular diseases, diabetes, cancer and even a variety of neurodegenerative diseases. Three proteins with potential bioactivities, including Neurohemerythrin, Hirudin and Eglin C, were selected as the quality makers and then quantified based on the characteristic peptides. CONCLUSIONS: This work profiled the proteome of three species of leeches, and addressed potential active proteins of the medicinal leech, which would help to provide the potential molecular mechanisms involved in disease treatment. The proteomics-based approach developed in this work is not only useful for the discovery of proteins with potential bioactivities but also helpful for the bioactivity relevant quality control of animal medicines.

Abstract aus PubMed (NCBI) für den zitierten Eintrag, in der Originalsprache wiedergegeben. Maßgeblich ist die Fassung der Originalpublikation.

Das Abstract wird nicht übersetzt, damit die eigene Wortwahl der Autoren erhalten bleibt.

Siebzehn Chargen getrockneter medizinischer Blutegel dreier Arten – Whitmania pigra (WP), Whitmania laevis (WL) und Poecilobdella manillensis (PM) – wurden per DNA-Barcoding authentifiziert und mittels markierungsfreier Proteomik analysiert. Die Autoren identifizierten 1 035 Proteine (452 in WP, 425 in WL, 158 in PM); 51 davon waren ausschließlich in einer Art exprimiert und Signalwegen wie PI3K-Akt, Wnt und HIF-1 zugeordnet. Hirudin, Eglin C und Neurohemerythrin quantifizierten sie als Qualitätsmarker. Der Ansatz diene der Entdeckung potenziell bioaktiver Proteine und der Qualitätskontrolle.

Erfahren Sie, wie diese Forschung mit der klinischen Praxis verknüpft ist

Weitere begutachtete Studien in der Kategorie „Genomik & Proteomik“.

• Leech Salivary Proteome and Transcriptome: 434 Deduced Protein Sequences — Liu Z, Tong X, Su Y, et al. (2019)
• Medicinal Leech Draft Genome — Anticoagulant Gene Survey — Kvist S, Manzano-Marín A, de Carle D, Trontelj P, Siddall ME (2020)
• Blutegeltherapie (Hirudo medicinalis) mildert die durch testikuläre Ischämie/Reperfusion induzierten Hodenschäden in einem Tiermodell — Davoodi F et al. (2021)
• Neues antikoagulatorisches Protein aus dem medizinischen Blutegel — Manuvera V et al. (2024)
• AIF-1 und RNASET2 spielen komplementäre Rollen in der angeborenen Immunantwort des medizinischen Blutegels — Baranzini N et al. (2019)
• Antimikrobielle Peptide des medizinischen Blutegels ohne Toxizität stellen eine vielversprechende alternative Strategie zur Bekämpfung antibiotikaresistenter Erreger dar — Grafskaia E et al. (2019)
• Structural insights into thrombolytic activity of destabilase from medicinal leech — Marin E et al. (2023)
• Das Genom des medizinischen Blutegels (Whitmania pigra) und eine vergleichende Genomstudie zur Erforschung bioaktiver Inhaltsstoffe — Tong L et al. (2022)
• Das ZNS des medizinischen Blutegels als Modell für Exosomen-Studien zum Wechselspiel zwischen Mikroglia und Neuronen — Raffo-Romero A et al. (2018)
• Transcriptomic profiling and the first spatial expression analysis of candidate genes in the salivary gland of the East Asian medicinal leech, Hirudo nipponia — Kim J et al. (2024)

Alle von der ASH analysierten Studien →