Surface modification with PEG and hirudin for protein resistance and thrombin neutralization in blood contact
Research article published in Colloids and surfaces. B, Biointerfaces (2010)
Abstract
In this work, we hypothesize that a surface modified with both polyethylene glycol (PEG) and hirudin may provide a non-fouling, thrombin-neutralizing surface suitable for blood contacting applications. With gold as a model substrate we used two different approaches to the preparation of such a surface: (1) a "direct" method in which PEG was conjugated to hirudin and the conjugate was then immobilized on the gold; (2) a "sequential" method in which PEG was immobilized on the gold and hirudin then attached to the immobilized PEG. The surfaces were characterized by water contact angle, ellipsometry and XPS. The biological properties were investigated by measuring protein adsorption (fibrinogen and thrombin) from buffer and plasma; thrombin inhibition was measured using a chromogenic substrate assay. Hirudin immobilization was found to be more efficient on surfaces prepared by the "direct" method. "Sequential" surfaces, however, despite having a lower density of hirudin, showed greater biological activity (thrombin binding and inhibition).
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
In this work, we hypothesize that a surface modified with both polyethylene glycol (PEG) and hirudin may provide a non-fouling, thrombin-neutralizing surface suitable for blood contacting applications.
Por qué esto importa para la hirudoterapia
Este estudio examinó si las superficies de oro comodificadas con polietilenglicol (PEG) y hirudina podrían resistir la adsorción de proteínas y neutralizar la trombina para posibles aplicaciones en biomateriales en contacto con la sangre, comparando dos estrategias de inmovilización. El trabajo caracteriza la actividad inhibidora de la trombina por parte de la hirudina en un contexto de ingeniería de superficies, lo cual es mecánicamente pertinente para la anticoagulación y la compatibilidad con la sangre, temas adyacentes al ámbito de la ASH. Sin embargo, el resumen describe la hirudina únicamente como un reactivo inmovilizado, sin ningún contexto de sanguijuela, salival o de hirudoterapia. El estudio se limita a la caracterización de superficies in vitro y a ensayos de adsorción de proteínas en sustratos de oro modelo, sin pruebas clínicas ni aplicaciones terapéuticas demostradas; su relevancia para la hirudoterapia es, por lo tanto, indirecta y como mucho conceptual.
Citación
Surface modification with PEG and hirudin for protein resistance and thrombin neutralization in blood contact.
Alibeik S, Zhu S, Brash J · Colloids and surfaces. B, Biointerfaces, 2010
Contexto clínico relacionado
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Añadido a la biblioteca ASH: March 18, 2026 · Última actualización del sitio: June 18, 2026