A novel RGD-toxin protein, Lj-RGD3, from the buccal gland secretion of Lampetra japonica impacts diverse biological activities
Comparative biochemistry published in Biochimie (2010)
Abstract
RGD (Arg-Gly-Asp) motif toxin proteins from snake venoms, saliva glands secretion of leech or tick have typical characteristics of inhibiting platelet aggregation, angiogenesis, and tumor growth. Here we report cloning and characterization of a novel RGD-toxin protein from the buccal gland of Lampetra japonica. In an attempt to study the activities of anticoagulant in the buccal gland secretion of L. japonica, we established buccal gland cDNA library and identified a gene encoding a predicted protein of 118 amino acids with 3 RGD motifs. The predicted protein was named Lj-RGD3. We generated the cDNA of Lj-RGD3 and obtained the recombinant protein rLj-RGD3. The polyclonal antibodies against rLj-RGD3 recognized the native Lj-RGD3 protein in buccal gland secretion in Western blot analyses. The biological function studies reveal that rLj-RGD3 inhibited human platelet aggregation in a dose-dependent manner with IC(50) value at 5.277 μM. In addition, rLj-RGD3 repressed bFGF-induced angiogenesis in the chick chorioallantoic membrane model. rLj-RGD3 also inhibited the adhesion of ECV304 cells to vitronectin. Furthermore, rLj-RGD3 induced apoptosis and significantly inhibited proliferation, migration, and invasion evoked by bFGF in ECV304 cells. Taken together, these results suggested that rLj-RGD3 is a novel RGD-toxin protein possessing typical functions of the RGD-toxin protein.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Lamprey (Lampetra japonica) buccal-gland RGD-toxin protein Lj-RGD3 inhibits platelet aggregation (IC50 5.3 µM), represses bFGF-induced angiogenesis, induces apoptosis, and inhibits proliferation/migration of endothelial cells — drawing biochemical parallels with leech-derived RGD toxins.
Por qué esto importa para la hirudoterapia
Este estudio clonó y caracterizó Lj-RGD3, una nueva proteína toxina RGD con tres motivos RGD, a partir de la glándula bucal de Lampetra japonica. La rLj-RGD3 recombinante inhibió la agregación plaquetaria humana de forma dependiente de la dosis (IC50 5,277 μM), suprimió la angiogénesis inducida por bFGF en el modelo de membrana corioalantoidea de pollo, e inhibió la adhesión, proliferación, migración e invasión de células ECV304. El resumen señala que proteínas toxina con motivo RGD con actividades inhibitorias similares se encuentran en las secreciones salivales de sanguijuelas y garrapatas, proporcionando un contexto de antecedentes. Sin embargo, el estudio en sí involucra a Lampetra japonica, no a sanguijuelas, y no realiza afirmaciones directas sobre la hirudoterapia ni sobre el secretoma de la sanguijuela; su relevancia para el dominio de ASH es limitada.
Citación
A novel RGD-toxin protein, Lj-RGD3, from the buccal gland secretion of Lampetra japonica impacts diverse biological activities.
Wang J et al. · Biochimie, 2010
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Añadido a la biblioteca ASH: May 26, 2026 · Última actualización del sitio: 18 de junio de 2026