Hirudins and fenestrins of the African medicinal leech Asiaticobdella fenestrata

African hematophagous leeches are largely underinvestigated both in terms of their biodiversity and the characterization of the repertoire of bioactive components of their salivary gland secretions. Asiaticobdella (formerly Aliolimnatis) fenestrata Moore, 1939, also referred to as the African medicinal leech, is hence a promising candidate for the identification and functional characterization of putative anticoagulants that may serve as inspirations for the development of new or the enhancement of existing drugs for medical purposes. A. fenestrata inhabits flooded plains of southern African regions and mainly feeds on mammals like black lechwe (Kobus leche Gray, 1850). Whereas several anticoagulants, including two putative hirudins of A. fenestrata, have already been identified in previous investigations, functional characterizations of these components are yet missing. We conducted an in-depth re-analysis of the salivary gland transcriptomic data set of A. fenestrata and identified two additional hirudin-like factors that exhibit a remarkable structural feature-the presence of an RGD motif at or near the N-terminal end of the molecules. The presence of such an RGD motif is a key feature of leech-derived platelet aggregation inhibitors such as ornatins and decorsins. However, both in ornatins and in decorsins, the respective RGD motif is located within the molecule between the 5th and the 6th cysteine residues, but not at or near the N-terminus. We named the two putative platelet aggregation inhibitors "fenestrins." Both the two putative hirudins and the two fenestrins of A. fenestrata were expressed as recombinant proteins, purified, and functionally characterized in either coagulation or platelet aggregation assays.

Resumen tomado de PubMed (NCBI) para el registro citado, en el idioma original. La versión de referencia es la de la publicación original.

El resumen se deja sin traducir para conservar la redacción original de los autores.

Re-analysis of Asiaticobdella fenestrata salivary transcriptomes identified two additional hirudin-like factors bearing N-terminal RGD motifs, named fenestrins; these putative platelet-aggregation inhibitors, alongside previously known hirudins, were expressed recombinantly and characterized in coagulation and platelet-aggregation assays.

Explore cómo esta investigación se conecta con la práctica clínica

Más estudios revisados por pares en la categoría «Genómica y proteómica».

• Leech Salivary Proteome and Transcriptome: 434 Deduced Protein Sequences — Liu Z, Tong X, Su Y, et al. (2019)
• Medicinal Leech Draft Genome — Anticoagulant Gene Survey — Kvist S, Manzano-Marín A, de Carle D, Trontelj P, Siddall ME (2020)
• Effects of hirudotherapy on liver functions, lipid profile, and insulin sensitivity in rats with metabolic syndrome — Bilden A et al. (2026)
• Efficacy and Safety of Hirudotherapy for Improving Sperm Quality Parameters in Male Infertility: A Randomized Controlled Trial — Abtahi-Forooshani M et al. (2026)
• Hirudoterapia en la cicatrización de heridas — Nair H et al. (2022)
• Medicinal leeches and hirudotherapy — Gödekmerdan A et al. (2011)
• Cutaneous pseudolymphoma after hirudotherapy : Case report and review — Hanner S et al. (2022)
• Hirudoterapia en la presbiacusia precoz en liquidadores del accidente de Chernóbil durante los años posteriores a la catástrofe — Zabolotnyi D, Mishchanchuk N (2019)
• Hirudotherapy (medicinal leeches) for treatment of upper airway obstruction in a dog — Trenholme H, Masseau I, Reinero C (2021)
• Leech therapy — Yantis M, O'Toole K, Ring P (2009)

Ver toda la investigación analizada por la ASH →