Combination of quercetin, hirudin and cinnamaldehyde promotes Schwann cell differentiation and myelination against high glucose by inhibiting ERK signaling
Mechanism study published in Chinese Journal of Integrative Medicine (2020)
Abstract
OBJECTIVE: To investigate the therapeutic and synergistic effects of QHC (combination of quercetin (Q), hirudin (H) and cinnamaldehyd (C)) on Schwann cell differentiation and myelination against high glucose (HG) induced injury. METHODS: Primary-culture Schwann cells exposed to HG (50 mmol/L) for 72 h and Schwann cell-dorsal root ganglion (DRG) neuron cocultures exposed to HG (50 mmol/L) for 7 days were employed as in vitro model of diabetic neuropathy. The cells were randomly divided into 10 groups: control (CON, 25 mmol/L glucose), HG (50 mmol/L glucose), HG plus 10 μmol/L quercetin (Q), HG plus 0.04 IU/mL hirudin (H), HG plus 100 nmol/L cinnamaldehyd (C), HG plus 10 μmol/L quercetin and 0.04 IU/mL hirudin (QH), HG plus 10 μmol/L quercetin and 50 nmol/L cinnamaldehyd (QC), HG plus 0.04 IU/mL hirudin and 50 nmol/L cinnamaldehyd (HC), HG plus 10 μmol/L quercetin, 0.04 IU/mL hirudin and 50 nmol/L cinnamaldehyd (QHC) or 10 μmol/L U0126. Cell differentiation was evaluated by periaxin immunofluorescence staining. The protein expression levels of myelin protein zero (P0), myelin basic protein (MBP), myelin-associated glycoprotein (MAG), extracellular signal-regulated kinase (ERK), p-ERK, p-c-Jun, c-Jun, notch intracellular domain (NICD) and the mRNA expression levels of P0, MBP, MAG, Krox-20, Notch1 and Jagged1 were detected by Western blotting and real-time quantitative PCR analysis. The secretion of ciliary neurotrophic factor (CNTF) was determined by enzyme-linked immunosorbent assay (ELISA). The number and length of the myelin segments were evaluated by MBP immunofluorescence staining. The expression and the location of p-ERK in cocultures were detected by MAG and p-ERK immunofluorescence double staining. RESULTS: Co-treatment with Q, C, H and their combination promoted Schwann cell differentiation, increased CNTF secretion, up-regulated the protein and mRNA expressions of myelin, and increased the number and length of the myelin segments (P<0.01 or P<0.05). In particular, the combination therapy of Q, H and C was superior to the respective monotherapy (P<0.01). Combination therapy of QHC exhibited higher inhibitory activities for ERK signaling related molecules than each monomer or the combination of the two monomers (P<0.01). CONCLUSION: QHC combination yielded synergy in promoting Schwann cell differentiation and myelination and the protective effect may involve in the inhibition of ERK signaling pathway, providing scientific evidence for better understanding of combination of Q, H and C in clinical applications.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Combination of quercetin, hirudin and cinnamaldehyde promotes Schwann cell differentiation/myelination under high glucose via ERK signaling inhibition — diabetic neuropathy mechanism.
Por qué esto importa para la hirudoterapia
Este estudio in vitro examinó si una combinación de quercetina, hirudina y cinamaldehído (QHC) podía promover la diferenciación de células de Schwann y la mielinización en condiciones de alta glucosa que modelan la neuropatía diabética, utilizando cultivos primarios de células de Schwann y cocultivos de células de Schwann con neuronas de ganglio de la raíz dorsal. Los autores informan que QHC reguló al alza proteínas y ARNm mielínicos, aumentó la secreción de CNTF y favoreció la formación de segmentos de mielina en comparación con las monoterapias, con un efecto protector que posiblemente implica la inhibición de la vía de señalización ERK. La hirudina es uno de los tres componentes de la combinación, lo que otorga al estudio una conexión con la investigación sobre la hirudina como molécula bioactiva. Sin embargo, el estudio es enteramente in vitro, utiliza la hirudina como agente único dentro de una combinación y no en el contexto de la terapia con sanguijuelas completas ni del secretoma de la sanguijuela, y el resumen no aborda la traslación clínica ni el uso terapéutico de la hirudina más allá de este contexto experimental.
Citación
Combination of quercetin, hirudin and cinnamaldehyde promotes Schwann cell differentiation and myelination against high glucose by inhibiting ERK signaling.
Liu D et al. · Chinese journal of integrative medicine, 2020
Contexto clínico relacionado
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026