A study of the influence of lead pollution on the anticoagulant activity of Whitmania pigra based on pharmacodynamics and metabolomics research
Quality control study published in Journal of Chromatography B (2021)
Abstract
Whitmania pigra Whitman (leech, also called Shuizhi in China, abbreviated as SZ), which has been used as a traditional Chinese medicine in the treatment of blood stasis syndrome (BSS) for a long time, is vulnerable to lead pollution in aquaculture environments. SZ has good anticoagulant activity. However, there are few studies on the influence of lead pollution on it. Therefore, we carried out the following researches to explore the influence of lead pollution on the anticoagulant activity of SZ and its mechanism. Firstly, the acute blood stasis model of rats was established by subcutaneous injection of adrenaline hydrochloride and ice water bath. Then unpolluted SZ (UPS) and lead-polluted SZ (LPS) were extracted. Next, the blood stasis model rats were administrated by gavage and the rats in normal control (NC) group and blood stasis model (BM) group were given the same amount of normal saline. Finally, the blood of the rats was collected to detect the coagulation function and hemorheology indexes. The metabolomics of rat plasma was studied by ultra-high-performance liquid chromatography coupled with orbitrap mass spectrometry (UPLC-Orbitrap-MS) technology. Principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and Hierarchical clustering analysis (HCA) were used to perform metabolomics analysis. MetPA analysis was used to search for related metabolic pathways. The results of coagulation function and hemorheology showed that lead pollution could decrease the anticoagulant activity of SZ. The OPLS-DA score plots indicated that the plasma metabolites of rats in LPS group were close to BM group, while UPS group tended to be close to NC group both in the positive and negative ion mode. Hierarchical cluster analysis (HCA) suggested that UPS group and NC group were clustered into a branch, while LPS group and BM group were clustered into a branch. To sum up, lead pollution will reduce the anticoagulant activity of SZ. And lead pollution reduces the anticoagulant activity of SZ probably by influencing the metabolic pathways such as sphingolipid metabolism, amino acid metabolism and energy metabolism in rats.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Резюме
UPLC-Orbitrap-MS metabolomics in blood stasis model rats showed that lead pollution of aquaculture-grown Whitmania pigra significantly reduces its anticoagulant activity by altering sphingolipid metabolism, amino acid metabolism, and energy metabolism pathways.
Почему это важно для гирудотерапии
В данном исследовании изучено влияние загрязнения свинцом на антикоагулянтную активность Whitmania pigra (Шуйчжи) — пиявки, применяемой в традиционной китайской медицине при синдроме застоя крови, с использованием модели острого застоя крови у крыс, индуцированной адреналином/ледяной водой, и плазменной метаболомики на приборе UPLC-Orbitrap-MS. Результаты оценки коагуляционной функции и гемореологии показали, что загрязнённые свинцом W. pigra обладали сниженной антикоагулянтной активностью по сравнению с незагрязнёнными пиявками, а метаболомический анализ (PCA, OPLS-DA, HCA) показал, что загрязнённая свинцом группа кластеризовалась вблизи модели заболевания, тогда как незагрязнённые пиявки — вблизи группы нормального контроля, что указывает на вовлечённость путей метаболизма сфинголипидов, аминокислот и энергетического обмена. Для ASH данная работа подчёркивает, что экологическое загрязнение культивируемых медицинских пиявок может снижать качество терапевтического антикоагулянтного действия. Ограничение: результаты ограничены моделью застоя крови у крыс, не включают характеристику слюнного секретома пиявок и не затрагивают непосредственно гирудотерапию живыми пиявками.
Цитирование
A study of the influence of lead pollution on the anticoagulant activity of Whitmania pigra based on pharmacodynamics and metabolomics research.
Chen X et al. · Journal of Chromatography B, 2021
Связанный клинический контекст
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Добавлено в библиотеку ASH: May 27, 2026 · Последнее обновление сайта: 18 июня 2026 г.