Molecular Epidemiological Characteristics of Carbapenem Resistantfrom Hospital Wastewater.
Research article published in Infection and drug resistance (2024)
Abstract
BACKGROUND: Hospital wastewater (HWW) promotes the spread of carbapenem resistance genes (CRGs). Aeromonas carry a large number of CRGs in HWW, they may play a role as a suitable reservoir for CRGs, while resistomes in HWW are still poorly characterized regarding carbapenem resistant Aeromonas. Thus, the aim of the study was to evaluate the molecular epidemiological characteristics of carbapenem resistant Aeromonas in HWW. METHODS: A total of 33 carbapenem resistant Aeromonas were isolated from HWW. Antimicrobial susceptibility testing and polymerase chain reaction (PCR) were used to assess the antimicrobial resistance profiles. Molecular typing was performed using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and multilocus sequence typing (MLST). The horizontal transmission mode of bla KPC was explored through conjugation and transformation experiments. The stability of bla KPC-IncP-6 plasmids was assessed through plasmid stability and in vitro competition test. The PCR mapping method was used to investigate the structural diversity of bla KPC. RESULTS: The detection rates of bla KPC and cphA in Aeromonas were 97.0% and 39.4% respectively. Aeromonas caviae were grouped into 13 clusters by ERIC-PCR and 12 STs by MLST. Aeromonas veronii were grouped into 11 clusters by ERIC-PCR and 4 STs by MLST. 56.3% bla KPC were located on mobilizable IncP-6 plasmids. bla KPC-IncP-6 plasmid showed high stability and low cost fitness. CONCLUSION: Carbapenem resistant Aeromonas from HWW mainly carried bla KPC, which exhibited great structural diversity. Aeromonas might serve as reservoirs for bla KPC and bla KPC might spread mainly through transformation in HWW.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Hospital wastewater (HWW) promotes the spread of carbapenem resistance genes (CRGs).carry a large number of CRGs in HWW, they may play a role as a suitable reservoir for CRGs, while resistomes in HWW are still poorly characterized regarding carbapenem resistant.
Por qué esto importa para la hirudoterapia
Este estudio de epidemiología molecular aisló 33 cepas de Aeromonas resistentes a los carbapenémicos provenientes de aguas residuales hospitalarias y encontró una tasa de detección de blaKPC muy elevada, con muchos genes blaKPC localizados en plásmidos IncP-6 movilizables que eran estables e impusieron un bajo costo de aptitud (fitness cost), concluyendo que Aeromonas puede actuar como reservorio de blaKPC y puede propagarlo principalmente mediante transformación. Esto es directamente pertinente para la seguridad de la hirudoterapia debido a que Aeromonas es el simbionte dominante del intestino de la sanguijuela medicinal y la causa habitual de infecciones post-sanguijuela; la evidencia de que las Aeromonas ambientales portan y transmiten fácilmente genes de resistencia a los carbapenémicos subraya por qué no puede asumirse que la elección de antibióticos empíricos para las infecciones asociadas a sanguijuelas sea efectiva y por qué debe basarse en la resistencia local. La salvedad honesta es que se trata de aislados de aguas residuales hospitalarias estudiados por su transmisibilidad génica, no de cepas derivadas de sanguijuelas ni de infecciones clínicas por sanguijuelas, por lo que el vínculo con la hirudoterapia es por género y mecanismo en lugar de por observación directa.
Citación
Molecular Epidemiological Characteristics of Carbapenem Resistantfrom Hospital Wastewater.
Zhang Q et al. · Infection and drug resistance, 2024
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Añadido a la biblioteca ASH: May 28, 2026 · Última actualización del sitio: June 18, 2026