Identification and characterization of novel anticoagulant peptide with thrombolytic effect and nutrient oligopeptides from Whitmania pigra
Peptidomics study published in Amino Acids (2016)
Abstract
Natural and nutrient substances for cardiovascular disease are promising and capture researchers' minds. Two kinds of novel bioactive peptides (high Fischer's ratio oligopeptides and anticoagulant peptides) were obtained from Whitmania pigra protein via enzymatic hydrolysis. An oligopeptide (MW<874.0 Da) named as HF2 was obtained via chromatography purification procedures with a high Fischer's ratio of 31.92 ± 1.36 and low phenylalanine + tyrosine content of 0.98 ± 0.04 %. Another peptide (WA3-1), prepared by alcalase AF 2.4 L-catalyzed hydrolysis and then purified by DEAE Sepharose FF, gel Sephadex G-15 chromatography, exhibited high anticoagulant activity with prolonging significantly plasma clotting time on activated partial thromboplastin time, prothrombin time, thrombin time (p < 0.01) and powerful thrombolytic activity. Amino acid composition and MALDI-TOF/TOF MS analysis showed that WA3-1 contained 11 amino acids (MW: 1422.0 Da) with the sequence as NH2-His-Asp-Phe-Leu-Asn-Asn-Lys-Leu-Glu-Tyr-Glu-COOH. Abundant negatively charged amino acids in C-terminal, as well as the special residue Lys contribute to its anticoagulant capacity. This research provided a novel natural candidate for the manufacture of nutrient oligopeptides with high branched chain amino acid, and anticoagulant thrombolytic agent in pharmaceutical industry with helping prevent from thrombosis and related cardiovascular diseases.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Discovers a novel anticoagulant peptide with thrombolytic activity and nutrient oligopeptides rich in branched-chain amino acids in Whitmania pigra hydrolysates.
Por qué esto importa para la hirudoterapia
Este estudio aisló y caracterizó dos nuevos péptidos bioactivos a partir de proteína de Whitmania pigra mediante hidrólisis enzimática y purificación cromatográfica: un oligopéptido con alto índice de Fischer (HF2, MW < 874,0 Da, índice de Fischer 31,92 ± 1,36) y un péptido anticoagulante (WA3-1, MW 1422,0 Da, secuencia NH2-His-Asp-Phe-Leu-Asn-Asn-Lys-Leu-Glu-Tyr-Glu-COOH). WA3-1 prolongó significativamente el tiempo de tromboplastina parcial activada, el tiempo de protrombina y el tiempo de trombina (p < 0,01) y exhibió actividad trombolítica, contribuyendo a su capacidad anticoagulante los aminoácidos C-terminal con carga negativa y un residuo de Lys. Esto es relevante para el dominio de ASH ya que caracteriza nuevos péptidos anticoagulantes y trombolíticos a partir de una especie de sanguijuela (según la clasificación MeSH), identificando posibles componentes bioactivos de interés medicinal. Sin embargo, todos los datos de actividad provienen de ensayos de coagulación plasmática in vitro y análisis composicional; no se presentan datos de eficacia in vivo, farmacocinéticos ni de seguridad.
Citación
Identification and characterization of novel anticoagulant peptide with thrombolytic effect and nutrient oligopeptides from Whitmania pigra.
Ren Y et al. · Amino acids, 2016
Contexto clínico relacionado
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026