Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal delta-integration
Comparative study published in J Biotechnol (2001)
Abstract
Recombinant Saccharomyces cerevisiae strains were developed to overproduce an anticoagulant hirudin. The delta-sequences of the yeast retrotransposon Ty1 and URA3 were used as target sites for a hirudin expression cassette. High copy-number transformants were successfully selected using a dominant selection antibiotic, G418. The copy numbers of the hirudin expression cassette integrated into delta-sequences of the yeast chromosome ranged from five to ten copies per cell. Production of hirudin in the delta-integrated recombinant S. cerevisiae system increased over two-fold compared with the YEp-based episomal hirudin expression system. A linear relationship between the copy number of the hirudin expression cassette and hirudin expression level was observed up to 10 copies. The hirudin expression cassettes integrated into the yeast chromosome were stably maintained in non-selective culture conditions.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Recombinant Saccharomyces cerevisiae strains with up to 10 copies of hirudin expression cassette integrated into delta-sequences; expression increased over two-fold compared to YEp-based episomal system.
Por qué esto importa para la hirudoterapia
Este estudio desarrolló cepas recombinantes de Saccharomyces cerevisiae para sobreproducir hirudina anticoagulante mediante la integración de un casete de expresión de hirudina en las secuencias delta cromosómicas del retrotransposón Ty1 de la levadura. Se alcanzaron números de copia de cinco a diez por célula, produciendo más del doble de hirudina en comparación con un sistema de expresión episomal, con mantenimiento estable en condiciones no selectivas. El resumen describe la hirudina únicamente como un anticoagulante y no menciona sanguijuelas, el secretoma de la sanguijuela ni la hirudoterapia. No existe una conexión defendible con el dominio de ASH a partir de este resumen. Se trata de un estudio de bioproducción en levaduras sin datos clínicos ni in vivo.
Citación
Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal delta-integration.
Kim MD et al. · J Biotechnol, 2001
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026