Hirunorms are true hirudin mimetics. The crystal structure of human alpha-thrombin-hirunorm V complex
Research article published in Protein science : a publication of the Protein Society (1998)
Abstract
A novel class of synthetic, multisite-directed thrombin inhibitors, known as hirunorms, has been described recently. These compounds were designed to mimic the binding mode of hirudin, and they have been proven to be very strong and selective thrombin inhibitors. Here we report the crystal structure of the complex formed by human alpha-thrombin and hirunorm V, a 26-residue polypeptide containing non-natural amino acids, determined at 2.1 A resolution and refined to an R-factor of 0.176. The structure reveals that the inhibitor binding mode is distinctive of a true hirudin mimetic, and it highlights the molecular basis of the high inhibitory potency (Ki is in the picomolar range) and the strong selectivity of hirunorm V. Hirunorm V interacts through the N-terminal tetrapeptide with the thrombin active site in a nonsubstrate mode; at the same time, this inhibitor specifically binds through the C-terminal segment to the fibrinogen recognition exosite. The backbone of the N-terminal tetrapeptide Chg1"-Val2"-2-Nal3"-Thr4" (Chg, cyclohexyl-glycine; 2-Nal, beta-(2-naphthyl)-alanine) forms a short beta-strand parallel to thrombin main-chain residues Ser214-Gly219. The Chg1" side chain fills the S2 subsite, Val2" is located at the entrance of S1, whereas 2-Nal3" side chain occupies the aryl-binding site. Such backbone orientation is very close to that observed for the N-terminal residues of hirudin, and it is similar to that of the synthetic retro-binding peptide BMS-183507, but it is opposite to the proposed binding mode of fibrinogen and of small synthetic substrates. Hirunorm V C-terminal segment binds to the fibrinogen recognition exosite, similarly to what observed for hirudin C-termninal tail and related compounds. The linker polypeptide segment connecting hirunorm V N-and C-terminal regions is not observable in the electron density maps. The crystallographic analysis proves the correctness of the design and it provides a compelling proof on the interaction mechanism for this novel class of high potency multisite-directed synthetic thrombin inhibitors.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
A novel class of synthetic, multisite-directed thrombin inhibitors, known as hirunorms, has been described recently.
Por qué esto importa para la hirudoterapia
Este artículo presenta la estructura cristalina a 2,1 Å de la alfa-trombina humana complejada con hirunorm V, un polipéptido hirudinomimético sintético de 26 residuos. El resumen demuestra que hirunorm V reproduce el modo distintivo de unión de la hirudina —el tetrapéptido N-terminal que se une al sitio activo en una conformación de hebra β paralela no sustrato y el segmento C-terminal que ocupa el exositio de reconocimiento del fibrinógeno— logrando una potencia picomolar y alta selectividad. Para ASH, esto resulta pertinente como validación estructural del diseño racional de miméticos de hirudina, aprovechando directamente la interacción hirudina-trombina como plantilla. La advertencia es que hirunorm V es completamente sintético; el estudio es puramente estructural y no contiene material derivado de sanguijuela ni datos in vivo.
Citación
Hirunorms are true hirudin mimetics. The crystal structure of human alpha-thrombin-hirunorm V complex
De Simone G et al. · Protein science : a publication of the Protein Society, 1998
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026