Hemorrhagic, coagulant and fibrino(geno)lytic activities of crude venom and fractions from mapanare (Bothrops colombiensis) snakes
Research article published in Comparative biochemistry and physiology Toxicology & pharmacology (2007)
Abstract
Bothrops colombiensis venom from two similar geographical locations were tested for their hemostatic functions and characterized by gel-filtration chromatography and SDS-PAGE electrophoresis. The snakes were from Caucagua and El Guapo towns of the Venezuelan state of Miranda. Fibrino(geno)lytic, procoagulant, hemorrhagic, lethal activities, gel-filtration chromatography and SDS-PAGE profiles were analyzed and compared for both venoms. The highest hemorrhagic activity of 5.3 mug was seen in El Guapo venom while Caucagua venom had the lowest LD(50) of 5.8 mg/kg. Both venoms presented similar thrombin-like activity. El Guapo showed a factor Xa-like activity two times higher than Caucagua. Differences were observed in kallikrein-like and t-PA activities, being highest in El Guapo. Caucagua venom showed the maximum fibrin lysis. Both crude venom runs on Sephadex G-100 chromatography gave fraction SII with the high fibrinolytic activity. Proteases presented in SII fractions and eluted from Benzamidine-Sepharose (not bound to the column) provoked a fast degradation of fibrinogen alpha chains and a slower degradation of beta chains, which could possibly be due to a higher content of alpha fibrinogenases in these venoms. The fibrinogenolytic activity was decreased by metalloprotease inhibitors. The results suggested that metalloproteases in SII fractions were responsible for the fibrinolytic activity. The analysis of samples for fibrin-zymography of SII fractions showed an active band with a molecular mass of approximately 30 kDa. These results reiterate the importance of using pools of venoms for antivenom immunization, to facilitate the neutralization of the maximum potential number of toxins.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Резюме
Hemorrhagic, coagulant and fibrino(geno)lytic activities of crude venom and fractions from mapanare (Bothrops colombiensis) snakes.
Почему это важно для гирудотерапии
В данном исследовании анализировалась и сопоставлялась геморрагическая, коагулянтная и фибрин(оген)олитическая активность нативного яда змей Bothrops colombiensis, собранного в двух соседних городах Венесуэлы. Исследователи выявили различные уровни тромбин-подобной, фактора Xa-подобной и металлопротеазной фибринолитической активности среди географических образцов, в конечном итоге отметив важность использования пула ядов для иммунизации при производстве противоядий. Хотя исследование подробно рассматривает нарушения гемостаза и протеолитические ферменты, участвующие в свёртывании крови, оно строго сфокусировано на характеристике токсичности змеиного яда. Таким образом, в реферате не содержится информации, релевантной гирудотерапии, секретому пиявок или терапевтическому применению медицинских пиявок.
Цитирование
Hemorrhagic, coagulant and fibrino(geno)lytic activities of crude venom and fractions from mapanare (Bothrops colombiensis) snakes
Giron ME et al. · Comparative biochemistry and physiology Toxicology & pharmacology, 2007
Связанный клинический контекст
Узнайте, как это исследование связано с клинической практикой
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