Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia)
Proteomics study published in PLoS One (2024)
Abstract
Hirudo nipponia is an important medicinal animal in China. Its salivary gland secretions contain a variety of protein bioactive substances. Investigations of its salivary glands are of great significance in the study of the medicinal value and mechanism of leech secretions. Illumina RNA-Seq technology was used to perform transcriptome sequencing of salivary gland tissue of H. nipponia under starvation (D30) and fed (D0) states. A total of 2,650 differentially expressed genes (DEGs) were screened. Using the label-free protein quantification technique and bioinformatics analysis, the expression of differentially expressed proteins (DEPs) in the salivary gland tissue of H. nipponia was compared. A total of 2,021 proteins were identified, among which 181 proteins were differentially expressed between the starvation and fed states, with 72 significantly upregulated and 109 significantly downregulated. The salivary glands of H. nipponia synthesized protein-based active substances after 30 days of starvation and adapted to the starvation environment by weakening respiratory activity and reducing metabolic activity to reduce energy expenditure. Energy was produced by glycolysis and the tricarboxylic acid cycle for the synthesis of substances such as antibiotics. This study combined transcriptome and proteome sequencing data to provide a data reference for an in-depth study of the regulatory mechanism of salivary gland secretions of H. nipponia under starvation stress by analyzing DEGs and DEPs.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Transcriptome and label-free proteomics of Hirudo nipponia salivary glands identified 2,650 DEGs and 181 differentially expressed proteins between starvation (30 day) and fed states.
Por qué esto importa para la hirudoterapia
Este estudio examina los cambios inducidos por el ayuno en el proteoma y transcriptoma de las glándulas salivales de la sanguijuela Hirudo nipponia, una especie medicinal importante en China. Mediante RNA-Seq de Illumina y cuantificación de proteínas label-free, los investigadores identificaron 2.650 genes diferencialmente expresados y 181 proteínas diferencialmente expresadas entre los estados de ayuno (30 días) y alimentado, revelando que las glándulas salivales en ayuno continuaban sintetizando sustancias activas de base proteica al tiempo que reducían la actividad metabólica. Esto es directamente relevante para el ámbito de la ASH, ya que amplía el conocimiento sobre cómo el secretoma de la sanguijuela —la fuente de compuestos bioactivos fundamentales para la hirudoterapia— responde al estrés fisiológico. Sin embargo, este es un estudio preclínico de biología molecular centrado en el perfil de expresión génica y proteica bajo condiciones de ayuno, sin medición directa de la eficacia terapéutica ni de los resultados clínicos.
Citación
Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia).
Cai M et al. · PLoS One, 2024
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026