Sociedad Americana de Hirudoterapia

Neurite-stimulating activity of secretions from the Hirudo medicinalis salivary gland in cultured sensory neurons

Basic science published in Ross Fiziol Zh (2001)

Última actualización: June 18, 2026Revisado por: ASH Editorial Board
Research article — evidence reviewArticle reference
Evidence: Preclinical (animal)Farmacología salivalChalisova NI et al. · Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 2001

Abstract

Components of leech (Hirudo medicinalis) salivary gland secretion (high molecular mass bdelin B, bdellastasin, eglin C, destabilize) were used to investigate their neurotrophic effect on organotypic culture of dorsal root ganglia of the 10-11-day old chicken embryos. Destabilize and high molecular mass bdelin B in concentrations of 0.01, 0.02, 0.05 and 0.1 ng/ml, bdellastasin in concentrations of 0.02 and 0.05 ng/ml, eglin C in concentrations of 0.1 ng/ml exhibit neurite-stimulating effect as compared to the control.

Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.

Publication typeEnglish AbstractJournal Article
Indexed MeSH termsAnimalsChick EmbryoEndopeptidasesGanglia, SpinalInsect ProteinsLeechesMolecular WeightNeuritesNeurons, AfferentOrganic ChemicalsProtease InhibitorsProteins

Resumen

Bdellin B, bdellastasin, eglin C, and destabilase at nanogram-per-mL concentrations exert neurite-stimulating effects on chick embryo dorsal root ganglia.

Por qué esto importa para la hirudoterapia

This laboratory study investigated the neurotrophic effects of four purified components of Hirudo medicinalis salivary gland secretion—high molecular mass bdelin B, bdellastasin, eglin C, and destabilize—on organotypic cultures of dorsal root ganglia from 10-11-day-old chicken embryos. Destabilize and high molecular mass bdelin B (0.01-0.1 ng/ml), bdellastasin (0.02 and 0.05 ng/ml), and eglin C (0.1 ng/ml) exhibited neurite-stimulating effects compared to control. This is directly relevant to ASH's domain, identifying specific leech secretome components with potential neurotrophic bioactivity. The caveat is that this is an in vitro organotypic culture study in avian sensory neurons, with no data on mammalian systems, in vivo effects, or therapeutic applicability to human neurological conditions.

Citación

Neurite-stimulating activity of secretions from the Hirudo medicinalis salivary gland in cultured sensory neurons.

Chalisova NI et al. · Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 2001

DOIPubMed

Contexto clínico relacionado

Explore cómo esta investigación se conecta con la práctica clínica

Salivary Gland SecretionAnti-Inflammatory MechanismsSalivary ComplexDestabilaseBdellinsProteinase InhibitorsEglinsNervous SystemNeurotrophic EffectsOtolaryngology

Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: June 18, 2026

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