A rapid method for isolation and purification of an anticoagulant from Whitmania pigra
Biochemistry/pharmacology published in Biomed Chromatogr (2007)
Abstract
Whitmania pigra is common in China and has been used as a traditional Chinese anticoagulant medicine for years, but its effective components are unknown to scientists. In this article we report a rapid method for isolation and purification of an anticoagulant from W. pigra for the first time. An acetone-water extract of W. pigra was subjected to anion-exchange chromatography on a Sephadex DEAE A-50 column, and gel permeation chromatography on Sephadex G-25 and Sephadex LH-20 columns successively, which afforded a fraction with potent anticoagulant activity. An anticoagulant was isolated and purified from this fraction by reversed-phase high-performance liquid chromatography (RP-HPLC). It was identified as a single pure substance by RP-HPLC and sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). This component was named whitmanin and its molecular weight was determined as 8608 Da by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS).
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
First isolation of 'whitmanin', an 8,608 Da anticoagulant protein purified by anion-exchange, gel-permeation, and reversed-phase HPLC from Whitmania pigra acetone-water extract.
Por qué esto importa para la hirudoterapia
Este estudio describe un método rápido para aislar y purificar un anticoagulante a partir de *Whitmania pigra*, una especie utilizada en la medicina tradicional china. Mediante diversas técnicas cromatográficas, los investigadores obtuvieron una fracción con potente actividad anticoagulante, de la cual purificaron con éxito una sustancia única denominada whitmanin. El compuesto se identificó como puro y su peso molecular se determinó en 8608 Da mediante espectrometría de masas. Si bien esta investigación identifica un compuesto anticoagulante específico a partir de una especie de sanguijuela, el alcance del resumen se limita estrictamente a la extracción y caracterización fisicoquímica, sin aportar datos sobre modelos in vivo, eficacia clínica ni sobre su uso en la hirudoterapia en vivo.
Citación
A rapid method for isolation and purification of an anticoagulant from Whitmania pigra.
Zhong S et al. · Biomedical chromatography : BMC, 2007
Contexto clínico relacionado
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Añadido a la biblioteca ASH: May 26, 2026 · Última actualización del sitio: 18 de junio de 2026