Oversulfated chondroitin sulfate binds to chemokines and inhibits stromal cell-derived factor-1 mediated signaling in activated T cells
Research article published in PloS one (2014)
Abstract
Oversulfated chondroitin sulfate (OSCS), a member of the glycosaminoglycan (GAG) family, was a contaminant in heparin that was linked to the 2008 heparin adverse events in the US. Because of its highly negative charge, OSCS can interact with many components of the contact and immune systems. We have previously demonstrated that OSCS inhibited the complement classical pathway by binding C1 inhibitor and potentiating its interaction with C1s. In the present study, by using surface plasmon resonance, we found OSCS interacts with T cell chemokines that can impact adaptive immunity. The binding of OSCS to stromal cell-derived factor-1 (SDF-1) chemokines, SDF-1α and SDF-1β, caused a significant change in the secondary structures of these chemokines as detected by far-ultraviolet circular dichroism spectra analysis. Functionally, OSCS binding profoundly inhibited SDF-1-induced calcium mobilization and T cell chemotaxis. Imaging flow cytometry revealed T cell morphological changes mediated by SDF-1α were completely blocked by OSCS. We conclude that the OSCS, a past contaminant in heparin, has broad interactions with the components of the human immune system beyond the contact and complement systems, and that may explain, in part, prior OSCS-related adverse events, while suggesting potentially useful therapeutic applications for related GAGs in the control of inflammation.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Oversulfated chondroitin sulfate (OSCS), a member of the glycosaminoglycan (GAG) family, was a contaminant in heparin that was linked to the 2008 heparin adverse events in the US.
Por qué esto importa para la hirudoterapia
Este estudio demuestra que el condroitín sulfato sobre-sulfatado (OSCS), un contaminante previamente presente en la heparina, se une a quimiocinas de linfocitos T e inhibe significativamente la señalización mediada por el factor derivado de células estromales 1, la movilización de calcio y los cambios morfológicos en los linfocitos T. La investigación analiza los impactos inmunológicos y las vías de señalización afectadas por este contaminante, concluyendo que el OSCS presenta amplias interacciones con componentes del sistema inmunitario humano más allá de los sistemas de contacto y complemento. El estudio no involucra sanguijuelas, hirudoterapia ni ningún compuesto derivado de sanguijuelas. Por lo tanto, su relevancia para la American Society of Hirudotherapy es totalmente inexistente, ya que aborda exclusivamente la inmunología humana y los eventos adversos asociados con un contaminante farmacéutico.
Citación
Oversulfated chondroitin sulfate binds to chemokines and inhibits stromal cell-derived factor-1 mediated signaling in activated T cells
Zhou ZH et al. · PloS one, 2014
Contexto clínico relacionado
Explore cómo esta investigación se conecta con la práctica clínica
Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026