Characterisation of separated end hyaluronan oligosaccharides from leech hyaluronidase and evaluation of angiogenesis
Research article published in Carbohydr Polym (2016)
Abstract
Hyaluronan oligosaccharides (o-HAs), especially saturated o-HAs, have attracted intensive attention due to their potential applications in medical treatments. In this study, the hydrolysis process of leech hyaluronidase (LHase) towards the hyaluronan was investigated by HPLC and HPLC/ESI-MS. The proportions of hyaluronan tetrasaccharide (HA4) with hexasaccharide (HA6), end products, were illustrated to have a relationship with the amount of LHase. Higher yield of HA4 was achieved with higher activity of LHase. After optimisation of the packing resin and operation parameters (balanced pH, elution concentration, elution volume and elution flow rate), the highly pure HA4 and HA6 were efficiently separated and prepared by combining ion exchange Q-Sepharose Fast Flow and size exclusion column chromatography. Compared with o-HAs (average Mr of 4000 Da), HA4 and HA6 were demonstrated to show higher activity for promoting angiogenesis, which was similar with the corresponding HA4 and HA6 produced by bovine testicular hyaluronidase. The pure HA4 and HA6 that prepared from LHase will attract intensive studies and be used in potential applications in near future.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Pure HA4 and HA6 oligosaccharides separated from leech hyaluronidase hydrolysate; show higher angiogenic activity than larger oligosaccharides.
Por qué esto importa para la hirudoterapia
Este estudio investigó la hidrólisis de hialuronano por la hialuronidasa de sanguijuela (LHase), caracterizando los productos finales mediante HPLC y HPLC/ESI-MS, y optimizó la separación del tetrasacárido (HA4) y hexasacárido (HA6) altamente puros mediante cromatografía de intercambio iónico y exclusión por tamaño. HA4 y HA6 demostraron mayor actividad promotora de angiogénesis en comparación con oligosacáridos más grandes, similar a los productos correspondientes de la hialuronidasa testicular bovina. Esto es relevante para ASH ya que examina la actividad enzimática de un componente derivado de sanguijuelas y sus productos oligosacáridos bioactivos. ADVERTENCIA: El resumen no especifica el sistema experimental utilizado para la evaluación de angiogénesis, y no se describe ninguna aplicación terapéutica humana ni hirudoterapia; la relevancia médica señalada es prospectiva, como se indica en el resumen.
Citación
Characterisation of separated end hyaluronan oligosaccharides from leech hyaluronidase and evaluation of angiogenesis.
Lv M et al. · Carbohydr Polym, 2016
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026