Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers
Research article published in Journal of clinical medicine (2020)
Abstract
Platelet count, indices (mean volume, young-immature platelet fraction) and aggregation are widely used laboratory parameters to investigate primary hemostasis. We performed a systematic, thorough evaluation of the influence of the time-interval since blood draw from 20 healthy individuals and of the anticoagulation of collected blood on such parameters. Blood was anticoagulated with citrate, K2-ethylenediaminetetraacetic acid (EDTA) and hirudin and analyzed 5, 30, 60, 120 and 180 min after blood draw. Multiple electrode aggregometry (MEA) was performed with either hirudin (half-diluted with NaCl) or citrate samples (half-diluted with NaCl or CaCl2 3 mM). Platelet count and indices (Sysmex XN-20) were rather stable over time with EDTA blood. MEA results were lower with citrate blood than with hirudin blood; supplementation with calcium was partially compensatory. MEA results were also lower when performed less than 30 or more than 120 min after blood draw. Platelet clumping, quantitatively estimated with microscope examination of blood smears, was more important in hirudin blood than citrate or EDTA blood and could explain some of the differences observed between preanalytical variables. The results stress once more the importance of preanalytical variables in hemostasis laboratory testing. Decision thresholds based on those tests are only applicable within specific preanalytical conditions.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Platelet count, indices (mean volume, young-immature platelet fraction) and aggregation are widely used laboratory parameters to investigate primary hemostasis.
Por qué esto importa para la hirudoterapia
This study systematically evaluated how time interval since blood draw and anticoagulant choice (citrate, EDTA, or hirudin) affect platelet count, indices, and impedance aggregometry results in samples from 20 healthy volunteers. Hirudin was used as a blood collection anticoagulant and for multiple electrode aggregometry (MEA), with results showing that hirudin blood yielded higher aggregation values than citrate but also more platelet clumping, and that MEA results were time-dependent. The abstract discusses hirudin solely as an in vitro laboratory anticoagulant reagent and does not mention leeches, leech therapy, or the origin of hirudin; no defensible connection to hirudotherapy or the leech secretome is stated in this abstract.
Citación
Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers
Hardy M et al. · Journal of clinical medicine, 2020
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: June 18, 2026