Effect of ingestion on antithrombin activity in different tissues of Whitmania pigra
Basic science / pharmacology published in Zhongguo Zhong Yao Za Zhi (2018)
Abstract
To study the effects of ingestion on antithrombin activities in different tissues of Whitmania pigra, the salivary glands, ingluvies, intestine and muscle of adult leeches were weighted on the 1st d, 3rd d, 5th d, 7th d and 11th d after feeding, respectively, and meanwhile antithrombin activity was measured by antithrombin titration method. The results showed that the antithrombin activity of salivary glands in different stages was significantly higher than that in other tissues (P<0.05). The activity of antithrombin in muscle tissue increased initially and then decreased with the prolongation of the time after feeding, and the peak was observed on the 5th day after feeding (P<0.05). The activity of antithrombin in the salivary glands, gluvies and intestine were found the highest on the 1st day after feeding(P<0.05), and then gradually decreased with the prolongation of the time of stopping the diet. The total amount of antithrombin activity on the 5th day was increased by 49.5%, 73.5%, 69.1% and 126.0% comparing with the 1st, 3rd, 7th and 11th day after feeding, respectively (P<0.05). In summary, both the feeding behavior and the food can induce the secretion of anticoagulant substances in the salivary glands and the digestive tract. The total amount of antithrombin activity was the highest on the 5th day after feeding and the 5th day after feeding was suggested as harvesting time.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Pharmacological characterization of antithrombin activity across Whitmania pigra tissues (salivary glands, ingluvies, intestine, muscle) at days 1, 3, 5, 7, 11 post-feeding. Peak total activity day 5; recommended as optimal harvesting time.
Por qué esto importa para la hirudoterapia
Este estudio examinó la actividad antitrombínica en las glándulas salivales, el ingluvio, el intestino y el músculo de sanguijuelas Whitmania pigra en múltiples puntos temporales tras la alimentación. La actividad antitrombínica fue significativamente mayor en las glándulas salivales que en los demás tejidos a lo largo de todas las etapas, alcanzó su pico en el día 1 en las glándulas salivales, el ingluvio y el intestino, mientras que la actividad del tejido muscular alcanzó su pico en el día 5. La actividad antitrombínica total fue máxima en el día 5 post-alimentación, lo que los autores sugirieron como momento de recolección. Esto resulta relevante para el ámbito de la ASH, ya que caracteriza la actividad antitrombínica en una especie de sanguijuela e informa sobre el momento adecuado para recolectar material anticoagulante derivado de la sanguijuela. La advertencia honesta es que se trata de un estudio preclínico/de tejidos animales sin correlación clínica, no aborda los desenlaces terapéuticos de la aplicación de sanguijuelas, y el resumen no especifica el uso posterior previsto del material recolectado.
Citación
Effect of ingestion on antithrombin activity in different tissues of Whitmania pigra.
Wu MJ et al. · China journal of Chinese materia medica, 2018
Contexto clínico relacionado
Explore cómo esta investigación se conecta con la práctica clínica
Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026