El granulado del par medicinal de sanguijuela y ciempiés mejora la disfunción eréctil mediante la inactivación de la señalización CaSR/PLC/PKC en ratas con diabetes inducida por estreptozotocina

Erectile dysfunction (ED) is one of the significant complications of diabetes mellitus (DM), and CASR plays an important role in cellular antiapoptosis and NO production in the vascular endothelium by activating PKC. The present study was aimed to investigate the efficacy of Leech and Centipede Granules (LCG) through the CaSR/PLC/PKC signaling. Fifty male Sprague-Dawley rats were treated with streptozotocin to induce the DM model. After 10 weeks, an apomorphine test was used to confirm DMED. Rats with DMED were administrated with LCG and U73122 for 4 weeks. Fasting blood glucose, body weight, insulin and glucagon levels were measured. Erectile function in rats was assessed by apomorphine. Serums were measured using enzyme-linked immunosorbent assay and flow cytometry, and penile tissues were harvested for histologic and the expression of related targets analyses. After treatment, fasting blood glucose, body weight, insulin, glucagon levels, and erectile function were significantly ameliorated in the LCG groups. The LOX-1, NOX, and EMPs concentrations were significantly decreased with LCG treatment. LCG also continuously increased NO and decreased ET-1 content in penile tissues. LCG and U73122 administration also improved penile fibrosis by significantly decreasing VCAM-1, ICAM-1, and CD62P. The data also showed that LCG reduced the apoptosis level in the penis. Furthermore, the inhibited activation of the CaSR/PLC/PKC pathway was observed in DMED rats with LCG treatment. Collectively, LCG significantly ameliorated erectile function of DMED rats via increased NO generation, inhibiting endothelial cells apoptosis and penile fibrosis, which might benefit from the suppression of CaSR/PLC/PKC pathway in DMED rats.

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En 50 ratas Sprague-Dawley macho se indujo diabetes con estreptozotocina; a las 10 semanas la apomorfina confirmó DMED. Tras 4 semanas con LCG y U73122, glucemia en ayunas, peso, insulina, glucagón y función eréctil mejoraron significativamente; LOX-1, NOX, EMPs, VCAM-1, ICAM-1 y CD62P disminuyeron, el NO aumentó y la ET-1 bajó. LCG atenuó la apoptosis peneana e inhibió la vía CaSR/PLC/PKC. Los autores atribuyen la mejoría al aumento de NO, a la menor apoptosis endotelial y fibrosis peneana.

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