Американское общество гирудотерапии

A subtractive cDNA library from an identified regenerating neuron is enriched in sequences up-regulated during nerve regeneration

Genomics published in Invert Neurosci (1997)

Последнее обновление: June 18, 2026Рецензент: ASH Editorial Board
Research article — evidence reviewArticle reference
Evidence: Preclinical (animal)Геномика и протеомикаKorneev S et al. · Invertebrate neuroscience : IN, 1997

Abstract

We have constructed a subtractive cDNA library from regenerating Retzius cells of the leech, Hirudo medicinalis. It is highly enriched in sequences up-regulated during nerve regeneration. Sequence analysis of selected recombinants has identified both novel sequences and sequences homologous to molecules characterised in other species. Homologies include alpha-tubulin, a calmodulin-like protein, CAAT/enhancer-binding protein (C/EBP), protein 4.1 and synapsin. These types of proteins are exactly those predicted to be associated with axonal growth and their identification confirms the quality of the library. Most interesting, however, is the isolation of 5 previously uncharacterised cDNAs which appear to be up-regulated during regeneration. Their analysis is likely to provide new information on the molecular mechanisms of neuronal regeneration.

Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.

Publication typeJournal ArticleResearch Support, Non-U.S. Gov't
Indexed MeSH termsAmino Acid SequenceAnimalsBase SequenceCCAAT-Enhancer-Binding ProteinsCalmodulinCytoskeletal ProteinsDNA, ComplementaryDNA-Binding ProteinsGene LibraryLeechesMembrane ProteinsMolecular Sequence Data

Резюме

Subtractive cDNA library from regenerating Retzius cells of Hirudo medicinalis identified both novel sequences and homologs of known nerve-regeneration molecules from other species — foundational for leech neurogenomics.

Почему это важно для гирудотерапии

This study constructed a subtractive cDNA library from regenerating Retzius neurons of the medicinal leech Hirudo medicinalis, identifying sequences up-regulated during nerve regeneration including homologs of alpha-tubulin, calmodulin-like protein, C/EBP, protein 4.1, and synapsin, along with five novel uncharacterized cDNAs. These molecules are consistent with those expected in axonal growth pathways. The work contributes to understanding the molecular basis of neuronal regeneration in leeches, a topic of comparative neuroscience interest. However, this study has no direct relevance to hirudotherapy or the leech secretome; it is basic neurobiology research. ASH relevance is limited to the broader context of leech cell and molecular biology with no therapeutic implications.

Цитирование

A subtractive cDNA library from an identified regenerating neuron is enriched in sequences up-regulated during nerve regeneration.

Korneev S et al. · Invertebrate neuroscience : IN, 1997

DOI

Связанный клинический контекст

Узнайте, как это исследование связано с клинической практикой

Species IdentificationTaxonomySalivary Gland SecretionNervous SystemNeurotrophic EffectsOtolaryngology

Добавлено в библиотеку ASH: May 26, 2026 · Последнее обновление сайта: June 18, 2026

Этот сайт предоставляет образовательную информацию и не является медицинской консультацией, диагнозом или рекомендацией по лечению. Гирудотерапия сопряжена с клинически значимыми рисками и должна проводиться только квалифицированными клиницистами в рамках институционально утверждённых протоколов. Разрешение FDA 510(k) для медицинских пиявок ограничено определёнными показаниями; обсуждения исследовательского и нелицензионного применения отмечены соответствующим образом. Для индивидуальных медицинских рекомендаций обратитесь к квалифицированному медицинскому специалисту.