Американское общество гирудотерапии

Calin--a platelet adhesion inhibitor from the saliva of the medicinal leech

Research article published in Blood Coagul Fibrinolysis (1991)

Последнее обновление: June 18, 2026Рецензент: ASH Editorial Board
Research article — evidence reviewArticle reference
Evidence: Research reportФармакология секрета слюнных желёзРазработка лекарственных препаратовMunro R et al. · Blood Coagul Fibrinolysis, 1991

Abstract

The saliva of the medicinal leech, Hirudo medicinalis, contains a potent, hitherto unsuspected, inhibitor of collagen-mediated platelet adhesion/aggregation. Calin, of molecular size approximately 65,000 (reduced), has a rapid (1-10 min) effect on collagen which is reflected in its ability to suppress collagen-induced platelet aggregation, as well as adhesion of platelets to collagen-coated microcarrier beads. It also causes flocculation of Type I collagen fibril suspensions. Calin is differentiated from leech collagenase in two ways: (1) by demonstrating, by SDS-PAGE analysis of the products of incubations of Calin with Type I collagen at 37 degrees C, that Calin binds to but does not cleave collagen; and (2) by showing that Calin cannot be purified using the methods used to isolate leech collagenase. Calin's rapid and unusual interaction with collagen makes it a prime candidate for one of the agents that are the causative factors of the prolonged bleeding phenomenon seen after leech bites.

Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.

Publication typeJournal ArticleResearch Support, Non-U.S. Gov't
Indexed MeSH termsAnimalsCollagenHumansInvertebrate HormonesLeechesPlatelet AdhesivenessPlatelet AggregationPlatelet Aggregation InhibitorsProtein BindingProtein ConformationSalivaSalivary Proteins and Peptides

Резюме

Calin from Hirudo medicinalis saliva is a potent inhibitor of collagen-mediated platelet adhesion/aggregation with rapid effect on collagen; binds to but does not cleave collagen.

Почему это важно для гирудотерапии

This study identifies and characterizes Calin, an approximately 65,000 Da (reduced) protein from the saliva of Hirudo medicinalis that inhibits collagen-mediated platelet adhesion and aggregation. Calin rapidly interacts with collagen (1–10 min), suppresses collagen-induced platelet aggregation, inhibits platelet adhesion to collagen-coated microcarrier beads, and flocculates Type I collagen fibril suspensions; SDS-PAGE confirmed it binds but does not cleave collagen, distinguishing it from leech collagenase. For ASH, this is directly relevant as Calin is identified as a candidate causative factor for the prolonged bleeding phenomenon after leech bites. Caveat: This is an in vitro biochemical characterization with no in vivo, animal, or clinical data presented.

Цитирование

Calin--a platelet adhesion inhibitor from the saliva of the medicinal leech.

Munro R et al. · Blood Coagul Fibrinolysis, 1991

DOI

Связанный клинический контекст

Узнайте, как это исследование связано с клинической практикой

Salivary Gland SecretionAnti-Inflammatory MechanismsSalivary ComplexDrug PipelineDrug DevelopmentDirect Thrombin InhibitorsOtolaryngology

Добавлено в библиотеку ASH: May 27, 2026 · Последнее обновление сайта: June 18, 2026

Этот сайт предоставляет образовательную информацию и не является медицинской консультацией, диагнозом или рекомендацией по лечению. Гирудотерапия сопряжена с клинически значимыми рисками и должна проводиться только квалифицированными клиницистами в рамках институционально утверждённых протоколов. Разрешение FDA 510(k) для медицинских пиявок ограничено определёнными показаниями; обсуждения исследовательского и нелицензионного применения отмечены соответствующим образом. Для индивидуальных медицинских рекомендаций обратитесь к квалифицированному медицинскому специалисту.