Identification and characterization of a new member of snake venom thrombin inhibitors from Bothrops insularis using a proteomic approach
Research article published in Toxicon (2007)
Abstract
Snake venom C-type lectin-like proteins (CLPs) are ubiquitously found in Viperidae snake venoms and differ from the C-type lectins as they display different biological activities but no carbohydrate-binding activity. Previous analysis of the transcriptome obtained from the Bothrops insularis venom gland showed the presence of two clusters homologous to bothrojaracin (BJC) chains alpha and beta. In an effort to identify a new BJC-like molecule, we used an approach associated with proteomic technologies to identify the presence of the expressed protein and then to purify and characterize a new thrombin inhibitor from B. insularis venom. We also constructed homology models of this protein and BJC, which were compared with other C-type lectin-like family members and revealed several conserved features of this intriguing snake venom toxin family.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Identification and characterization of a new member of snake venom thrombin inhibitors from Bothrops insularis using a proteomic approach.
Por qué esto importa para la hirudoterapia
Este estudio utilizó un enfoque proteómico para identificar y caracterizar un nuevo inhibidor de trombina del veneno de la serpiente Bothrops insularis, identificado como una proteína similar a lectina de tipo C homóloga al bothrojaracin. Los investigadores purificaron con éxito la proteína expresada y construyeron modelos de homología molecular para comparar sus características estructurales con otras toxinas intrigantes del veneno de serpiente. Aunque la investigación se centra en el aislamiento de un inhibidor de trombina —una clase funcional que se paralela conceptualmente con las propiedades anticoagulantes del secretoma de la sanguijuela, como la hirudina—, la molécula se deriva enteramente del veneno de serpiente. En consecuencia, el estudio no presenta datos relacionados con sanguijuelas y no posee relevancia directa para la hirudoterapia clínica.
Citación
Identification and characterization of a new member of snake venom thrombin inhibitors from Bothrops insularis using a proteomic approach
Oliveira-Carvalho AL et al. · Toxicon, 2007
Contexto clínico relacionado
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026