An optimized method to assess in vivo efficacy of antithrombotic drugs using optical coherence tomography and a modified Doppler flow system
Research article published in Journal of pharmacological and toxicological methods (2011)
Abstract
INTRODUCTION: Animal models of venous and arterial thrombosis are extremely useful to study the efficacy of antithrombotic agents. Variability in efficacy data is often observed in those preclinical studies. The goal of this study was to optimize the methodology for assessing antithrombotic drug efficacy by the use of optical coherence tomography (OCT) and a modified Doppler flow system in rat models of thrombosis. METHODS: Thrombus formation was assessed in both the rat venous and arterial ferric chloride (FeCl(3)) models of thrombosis. In the venous model, thrombus volume post-treatment was measured using OCT, and data were correlated against the thrombus weight. In the arterial model, the time to occlusion was measured using a Doppler flow probe connected to a perivascular flow module which allowed the reporting of dynamic blood flow data every 30s. Heparin (130 or 165U/kg), argatroban (4.5mg/kg), bivalirudin (1.3mg/kg) or saline were administered intravenously. RESULTS: In the venous model, for all treatment groups a strong linear correlation (R(2)=0.998) was observed between thrombus volume measured by OCT and thrombus weight. In the arterial model, using a high sampling rate of a dynamic blood flow using a modified Doppler flow system provided data accuracy and precision of the time to occlusion measurement. DISCUSSION: This study demonstrates that OCT is a powerful tool for the assessment of antithrombotic drug efficacy. Furthermore, it shows that a high Doppler sampling rates of dynamic blood flow leads to data accuracy and precision.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Animal models of venous and arterial thrombosis are extremely useful to study the efficacy of antithrombotic agents.
Por qué esto importa para la hirudoterapia
Este estudio metodológico optimizó la evaluación de la eficacia de fármacos antitrombóticos mediante tomografía de coherencia óptica (OCT) y un sistema Doppler modificado en modelos de trombosis venosa y arterial inducida por FeCl₃ en ratas. Como tratamientos de prueba se administraron por vía intravenosa heparina, argatrobán, bivalirudina o solución salina; se demostró una fuerte correlación lineal (R²=0,998) entre el volumen de trombo medido por OCT y el peso del trombo en el modelo venoso, y el sistema Doppler modificado proporcionó una medición del tiempo hasta la oclusión precisa y exacta en el modelo arterial. No existe ningún vínculo defendible con las sanguijuelas o la hirudoterapia en este resumen: la bivalirudina se enumera entre los fármacos de prueba, pero el resumen no proporciona ninguna caracterización que la conecte con la hirudina o las sanguijuelas. El propósito del estudio es la validación de metodología de imagen para la evaluación antitrombótica preclínica.
Citación
An optimized method to assess in vivo efficacy of antithrombotic drugs using optical coherence tomography and a modified Doppler flow system
Couture L et al. · Journal of pharmacological and toxicological methods, 2011
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026