Counteracting effect of glycyrrhizin on the hemostatic abnormalities induced by Bothrops jararaca snake venom
Research article published in British journal of pharmacology (2006)
Abstract
1. Envenomation by the snake Bothrops jararaca is typically associated with hemostatic abnormalities including pro- and anticoagulant disturbances. Glycyrrhizin (GL) is a plant-derived thrombin inhibitor that also exhibits in vivo antithrombotic properties. Here, we evaluated the ability of GL to counteract the hemostatic abnormalities promoted by B. jararaca venom. 2. GL inhibited the human fibrinogen clotting (IC50 = approximately 1.0 mg ml(-1); 1.2 mM), H-D-phenylalanyl-L-pipecolyl-L-arginine-p-nitroanilide dihydrochloride hydrolysis (IC50 = approximately 0.4 mg ml(-1); 0.47 mM) and platelet aggregation (IC50 = approximately 0.28 mg ml(-1); 0.33 mM) induced by B. jararaca venom, in vitro. 3. The in vivo effect of GL was tested in rats using a model of venous thrombosis in which intravenous (i.v.) administration of B. jararaca venom (100 microg kg(-1)) produced in all animals a thrombus with a mean weight of 10.6+/-1.7 mg. 4. Prior administration of GL (180 mg kg(-1)) or antibothropic serum (27 microl kg(-1)) inhibited thrombus formation by 86 and 67%, respectively. Remarkably, co-administration of ineffective doses of GL and antibothropic serum markedly decreased thrombus weight, suggesting a synergistic effect. 5. Co-administration of GL with antibothropic serum abolished venom-induced bleeding. Ex vivo clotting times showed that rat plasma was non-clotting after i.v. administration of B. jararaca venom. Treatment with GL, antibothropic serum or both before venom administration efficiently prevented this abnormality. 6. Altogether, we demonstrate here that GL prevents both in vitro and in vivo venom-induced changes in hemostasis, suggesting a potential antiophidic activity.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Counteracting effect of glycyrrhizin on the hemostatic abnormalities induced by Bothrops jararaca snake venom.
Por qué esto importa para la hirudoterapia
Este estudio evaluó la glicirricina, un inhibidor de trombina de origen vegetal, por su capacidad para contrarrestar las alteraciones hemostáticas severas inducidas por el veneno de la serpiente Bothrops jararaca. Los investigadores encontraron que la glicirricina inhibió eficazmente la coagulación del fibrinógeno y la agregación plaquetaria inducidas por el veneno in vitro, mientras que previno la formación de trombos y el sangrado en modelos de rata cuando se coadministró con suero antibotrópico. Aunque el estudio examina un inhibidor de trombina exógeno con un mecanismo conceptualmente similar al de los anticoagulantes derivados de la sanguijuela como la hirudina, la glicirricina es estrictamente un compuesto vegetal probado con fines antiofídicos (antiveneno). Por lo tanto, el estudio carece de relevancia directa para la hirudoterapia o el secretoma de la sanguijuela.
Citación
Counteracting effect of glycyrrhizin on the hemostatic abnormalities induced by Bothrops jararaca snake venom
Assafim M et al. · British journal of pharmacology, 2006
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Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026