The effect and mechanism of freeze-dried powder ofon improving inflammatory injury of rat glomerular mesangial cells through TXNIP / NLRP3 pathway
Research article published in Heliyon (2024)
Abstract
OBJECTIVE: Diabetic kidney disease (DKD) is a common complication of diabetes mellitus. The pathophysiological changes in platelet function and the hypercoagulable state associated with DKD are closely linked to inflammatory processes. Poecilobdella manillensis (PM), a type of leech known for its anticoagulant and antithrombotic properties, has the potential to modulate the inflammatory response in DKD. This study aims to investigate the effect of freeze-dried powder of PM on improving inflammatory injury in rat glomerular mesangial cells and to explore its underlying mechanism. METHODS: Lipopolysaccharide (LPS) stimulated HBZY-1 rat mesangial cells to establish an in vitro DKD inflammation model. After the intervention with the water extract of freeze-dried powder of PM (FDPM), cell viability, NO content, and the levels of inflammatory factors such as IL-1β, IL-18, and TNF-α were assessed. Finally, utilizing transcriptomics technology, RT-qPCR, and Western blot methods, the mechanism by which FDPM improves inflammatory injury in rat glomerular mesangial cells was explored and preliminarily validated. RESULTS: FDPM effectively enhances cell viability and inhibits the production of NO and related inflammatory factors. Transcriptomic analysis suggests that FDPM may exert these effects by regulating the TXNIP/NLRP3 signaling pathway. The mRNA and protein expressions of TXNIP, NLRP3, and MCP-1 in the model cells were reversed by FDPM. CONCLUSION: FDPM may improve the micro-inflammatory state of DKD and slow the progression of the disease by regulating the TXNIP/NLRP3 signaling pathway. This study provides a scientific basis for the clinical application of PM DKD treatment.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Diabetic kidney disease (DKD) is a common complication of.
Por qué esto importa para la hirudoterapia
Este estudio examinó los efectos del polvo liofilizado de la sanguijuela Poecilobdella manillensis (FDPM) sobre la lesión inflamatoria en células mesangiales glomerulares de rata estimuladas con LPS, un modelo in vitro de enfermedad renal diabética. Los hallazgos indican que FDPM mejora la viabilidad celular y reduce los marcadores inflamatorios mediante la modulación de la vía de señalización TXNIP/NLRP3. Esto es altamente relevante para la American Society of Hirudotherapy, ya que explora las propiedades antiinflamatorias del extracto de una especie específica de sanguijuela, ampliando el potencial terapéutico conocido de las sustancias derivadas de sanguijuelas. Una advertencia importante es que estos resultados son estrictamente preclínicos, basados en un modelo in vitro de células de rata, y no establecen la eficacia clínica en pacientes humanos.
Citación
The effect and mechanism of freeze-dried powder ofon improving inflammatory injury of rat glomerular mesangial cells through TXNIP / NLRP3 pathway
Sun X et al. · Heliyon, 2024
Contexto clínico relacionado
Explore cómo esta investigación se conecta con la práctica clínica
Añadido a la biblioteca ASH: May 27, 2026 · Última actualización del sitio: 18 de junio de 2026