Comparative Study of Lefaxin Family in Two Asian Leeches: Hirudinaria manillensis and Whitmania pigra
Ye T, Zhao F, Xiao M, Yin J, Ai R, Tang L, Liu Z, Huang Z, Lin G (2025) · Biology · n=0
Характеристика исследования
- Дизайн
- genomic and transcriptomic sequencing of wild populations of Hirudinaria manillensis and Whitmania pigra with recombinant expression of lefaxin proteins and in-vitro Factor Xa anticoagulant activity assays (Chinese consortium led by Jinggangshan University)
- Размер выборки (n)
- —
- Вмешательство
- Comparative characterization of three lefaxin genes per species, plus recombinant lefaxin protein expression and in-vitro anticoagulant assays
- Компаратор
- Inter-species comparison (hematophagous H. manillensis vs non-hematophagous W. pigra)
- Первичная конечная точка
- Lefaxin protein anticoagulant activity (Factor Xa binding, in-vitro coagulation prolongation)
- Основной результат
- Hirudinaria manillensis lefaxins bound Factor Xa more effectively; Whitmania pigra lefaxins showed more robust in-vitro anticoagulant activity despite W. pigra being non-hematophagous; H. manillensis exhibited higher genetic diversity and stability; finding challenges traditional view that non-hematophagous leech species lack potent anticoagulants
- Длительность наблюдения
- Not applicable - in-vitro biochemistry
- PMID
- 40906077
Ключевые выводы
- Three lefaxin genes per species (LefH1-3 in H. manillensis, LefW1-3 in W. pigra)
- W. pigra lefaxins more potent in-vitro despite non-hematophagous ecology
- H. manillensis lefaxins better Factor Xa binders
- Provides recombinant protein expression workflow for both species
- Challenges traditional view that only blood-feeders have potent anticoagulants
Ограничения
- In-vitro biochemistry only — no in-vivo or clinical efficacy
- Recombinant proteins may differ from native molecules in folding or post-translational modification
- Limited population sampling
- No comparison to gold-standard recombinant hirudin or bivalirudin
- No mammalian pharmacokinetic or safety data
Клиническое значение
Ye 2025 deepens the comparative pharmacology of underrepresented Asian medicinal-leech species and demonstrates that Whitmania pigra lefaxins are clinically interesting anticoagulant candidates despite the species' non-hematophagous ecology. For ASH, the finding reinforces that 'medicinal leech' pharmacology is species-heterogeneous and that K040187 US clinical practice should not be conflated with global research on Asian Hirudinaria, Whitmania, or Haemadipsa species. Translational drug-discovery relevance only.
Связанные исследования
Hirudins and hirudin-like factors in Hirudinidae: implications for function and phylogenetic relationships
Müller C, Haase M, Lemke S, Hildebrandt JP (2016)
Diversity of hirudin and hirudin-like factor genes in the North-African medicinal leech, Hirudo troctina
Ahmed RB, Abilov A, Müller C (2024)
Monomeric and Oligomeric Decorsins of the Asian Medicinal Leech
Tolksdorf C, Wolf R, Rauch BH, Jedlitschky G, Müller C (2025)
Molecular Insights into Leech-Derived Bioactive Compounds: Biochemical Mechanisms and Therapeutic Potential
Raghavi S, Deva Darshini B, Saravanan KM, Anbarasu K (2026)