Immunohistochemical demonstration of peptides, serotonin and dopamine-beta-hydroxylase-like material in the nervous system of the leech Hirudo medicinalis.
Research article published in Histochemistry (1982)
Abstract
The central ganglia of the leech, Hirudo medicinalis, were processed for the immunohistochemical localisation of bombesin-, substance P-, cholecystokinin-, vasoactive intestinal polypeptide-, enkephalin-, serotonin- and dopamine-beta-hydroxylase-related substances. To varying extents all of the substances were localised in neuropile processes, and all, with the exception of substance P, were associated with specific perikarya. The most prominent neuropeptides, in terms of the number of immunoreactive neurones, were cholecystokinin and vasoactive intestinal peptide. The dopamine-beta-hydroxylase positive neurones are thought to be octopaminergic, and the serotonin monoclonal antibody revealed positive staining in the Retzius cells. We were unable to demonstrate the coexistence of pairs of substances in any neurones in the leech ganglia.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
The central ganglia of the leech, Hirudo medicinalis, were processed for the immunohistochemical localisation of bombesin-, substance P-, cholecystokinin-, vasoactive intestinal polypeptide-, enkephalin-, serotonin- and dopamine-beta-hydroxylase-related substances. To varying extents all of the...
Por qué esto importa para la hirudoterapia
Este estudio inmunohistoquímico localizó múltiples neuropéptidos y neurotransmisores —incluidos bombesina, sustancia P, colecistocinina, polipéptido intestinal vasoactivo, encefalina, serotonina y dopamina-beta-hidroxilasa— en los ganglios centrales de Hirudo medicinalis, identificando CCK y VIP como los más prominentes por número de neuronas, serotonina en las características células de Retzius, y presuntas neuronas octopaminérgicas. Este trabajo es directamente relevante para el ámbito de la ASH, ya que caracteriza la anatomía neuroquímica de la especie de sanguijuela medicinal central para la hirudoterapia. El estudio aporta la neurobiología fundamental de H. medicinalis, aunque no aborda la composición del secretoma, las aplicaciones terapéuticas ni los desenlaces clínicos de la hirudoterapia.
Citación
Immunohistochemical demonstration of peptides, serotonin and dopamine-beta-hydroxylase-like material in the nervous system of the leech Hirudo medicinalis.
Osborne et al. · Histochemistry, 1982
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Añadido a la biblioteca ASH: May 28, 2026 · Última actualización del sitio: 18 de junio de 2026