High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers
Research article published in Scientific reports (2014)
Abstract
Hyaluronidases (HAases), particularly leech HAases, have attracted intense attention due to their broad applications in medical treatments and great potential for the enzymatic production of hyaluronan oligosaccharides. However, little is known about this third interesting family of HAases. Here, we applied the random amplification of cDNA ends polymerase chain reaction (RACE-PCR) approach to identify the first leech HAase-encoding gene. By combining protein engineering and high-density culture, we achieved high-level production (8.42 × 10(5) U ml(-1)) in the yeast Pichia pastoris secretory expression system. Compared with the commercial bovine testicular HAase, the recombinant leech HAase exhibited superior enzymatic properties. Furthermore, analysis of the hydrolytic process suggested that this novel enzyme adopts a nonprocessive endolytic mode, yielding a narrow-spectrum of specific HA oligosaccharides with different incubation times. Large-scale production of this novel leech HAase will not only greatly promote medical applications but also facilitate the enzymatic production of specific HA oligosaccharides.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Resumen
Hyaluronidases (HAases), particularly leech HAases, have attracted intense attention due to their broad applications in medical treatments and great potential for the enzymatic production of hyaluronan oligosaccharides.
Por qué esto importa para la hirudoterapia
Este estudio identificó el primer gen que codifica hialuronidasa de sanguijuela mediante RACE-PCR y logró una producción recombinante de alto nivel (8,42 × 10⁵ U/ml) en la levadura Pichia pastoris. La hialuronidasa recombinante de sanguijuela exhibió propiedades enzimáticas superiores en comparación con la hialuronidasa testicular bovina comercial y operó mediante un modo endolítico no procesivo, generando oligosacáridos de hialuronano específicos con diferentes tiempos de incubación. Para el dominio de la ASH, este trabajo es relevante porque caracteriza una enzima derivada de sanguijuela con reconocidas aplicaciones médicas amplias, contribuyendo a la comprensión de los componentes enzimáticos del secretoma de la sanguijuela. Advertencia: se trata de un estudio de biotecnología centrado en la producción y caracterización de enzimas; no involucra hirudoterapia, aplicación clínica ni resultados terapéuticos en pacientes.
Citación
High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers.
Jin P et al. · Scientific reports, 2014
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Añadido a la biblioteca ASH: March 18, 2026 · Última actualización del sitio: June 18, 2026