Hirudins and fenestrins of the African medicinal leech Asiaticobdella fenestrata
Schulz L, Tolksdorf C, Rauch BH, Kvist S, Müller C (2025) · Parasitology Research · n=0
RCT-EvidenzdetailsStudienreferenz
Studienprofil
- Design
- comparative salivary gland transcriptome analysis of African medicinal leech Asiaticobdella fenestrata with recombinant protein expression and functional characterization
- Stichprobengröße (n)
- —
- Intervention
- Identification of two putative hirudins and two novel RGD-motif platelet aggregation inhibitors ("fenestrins") from A. fenestrata salivary glands
- Komparator
- Established hirudin and decorsin/ornatin sequences from European medicinal leech (Hirudo medicinalis)
- Primärer Endpunkt
- Structural and functional characterization of novel bioactive compounds in coagulation and platelet aggregation assays
- Primäres Ergebnis
- Two putative hirudins and two novel "fenestrins" (RGD-motif platelet aggregation inhibitors with unique N-terminal location) functionally characterized; demonstrates African leech species as untapped source of bioactive anticoagulant inspiration
- Follow-up-Dauer
- Laboratory characterization phase
- PMID
- 41198932
Wichtigste Ergebnisse
- Two novel hirudin-like factors with unique N-terminal RGD motif
- Two new platelet aggregation inhibitors named "fenestrins"
- Distinct from European hirudin/decorsin structural patterns
- Recombinant expression and functional characterization completed
- Extends understanding of leech biodiversity beyond H. medicinalis
Einschränkungen
- Preclinical characterization only - no clinical application yet
- Limited to A. fenestrata species
- RGD motif location significance not fully elucidated
- Functional assays in vitro only
- No bridge to clinical use established
Klinische Implikationen
Schulz 2025 expands the leech bioactive compound landscape by characterizing novel hirudins and fenestrins from the African medicinal leech Asiaticobdella fenestrata. For ASH, this preclinical work extends the species and compound diversity beyond European Hirudo medicinalis (the K040187-cleared species), informing future drug development for synthetic recombinant anticoagulants distinct from device-leech practice.
Verwandte Studien
Hirudins and hirudin-like factors in Hirudinidae: implications for function and phylogenetic relationships
Müller C, Haase M, Lemke S, Hildebrandt JP (2016)
Diversity of hirudin and hirudin-like factor genes in the North-African medicinal leech, Hirudo troctina
Ahmed RB, Abilov A, Müller C (2024)
Monomeric and Oligomeric Decorsins of the Asian Medicinal Leech
Tolksdorf C, Wolf R, Rauch BH, Jedlitschky G, Müller C (2025)
Molecular Insights into Leech-Derived Bioactive Compounds: Biochemical Mechanisms and Therapeutic Potential
Raghavi S, Deva Darshini B, Saravanan KM, Anbarasu K (2026)