Transcriptomic analysis of the salivary gland of medicinal leech Hirudo nipponia
Research article published in PloS one (2018)
Abstract
Hirudo nipponia (known as Shui Zhi in Chinese) is a well-known Chinese medicine with numerous active ingredients in its body, especially in its saliva. This native Chinese blood-sucking leech has been used for therapeutic purposes since before 100 AD. Modern Chinese physicians use it for a wide range of diseases. Genomic data and molecular information about the pharmacologically active substances produced by this medicinal leech are presently unavailable despite this organism's medicinal importance. In this study, we performed transcriptome profiling of the salivary glands of medicinal leech H. nipponia using the Illumina platform. In total, 84,657,362 clean reads were assembled into 50,535 unigenes. The obtained unigenes were compared to public databases. Furthermore, a unigene sequence similarity search and comparisons with the whole transcriptome of medical leech were performed to identify potential proteins. Finally, more than 21 genes were predicted to be involved in anticoagulatory, antithrombotic, antibacterial, anti-inflammatory and antitumor processes, which might play important roles in the treatment of various diseases. This study is the first analysis of a sialotranscriptome in H. nipponia. The transcriptome profile will shed light on its genetic background and provide a useful tool to deepen our understanding of the medical value of H. nipponia.
Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.
Summary
Hirudo nipponia (known as Shui Zhi in Chinese) is a well-known Chinese medicine with numerous active ingredients in its body, especially in its saliva.
Why This Matters for Hirudotherapy
This study performed Illumina-based transcriptome profiling of the salivary glands of Hirudo nipponia, a medicinal leech long used in Chinese traditional medicine, assembling over 50,000 unigenes and identifying more than 21 genes predicted to be involved in anticoagulant, antithrombotic, antibacterial, anti-inflammatory, and antitumor processes. This work is directly relevant to hirudotherapy and the leech salivary secretome, as it provides the first sialotranscriptome catalog for H. nipponia and nominates candidate bioactive molecules that may underlie the therapeutic effects of leech saliva, potentially guiding future functional characterization. However, the gene identifications are computational predictions based on sequence similarity searches, with no functional validation, protein-level confirmation, or pharmacological outcome data presented. The relevance to actual therapeutic applications remains indirect and requires downstream experimental verification of these candidate genes' biological activities.
Citation
Transcriptomic analysis of the salivary gland of medicinal leech Hirudo nipponia.
Lu Z et al. · PloS one, 2018
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