American Society of Hirudotherapy

Characterization of the digestive-tract microbiota of Hirudo orientalis, a european medicinal leech

Microbiology article published in Applied and Environmental Microbiology (2008)

Last Updated: June 18, 2026Reviewed by: ASH Editorial Board
Research article — evidence reviewArticle reference
Evidence: Preclinical (animal)Genomics & ProteomicsSafety & Infection ControlLaufer AS, Siddall ME, Graf J · Applied and environmental microbiology, 2008

Abstract

FDA-approved, postoperative use of leeches can lead to bacterial infections. In this study, we used culture-dependent and culture-independent approaches to characterize the digestive-tract microbiota of Hirudo orientalis. Surprisingly, two Aeromonas species, A. veronii and A. jandaei, were cultured. Uncultured Rikenella-like bacteria were most similar to isolates from Hirudo verbana.

Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.

Publication typeJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.
Indexed MeSH termsAnimalsBacteriaDNA, BacterialDNA, RibosomalEuropeGastrointestinal TractGenes, rRNALeechesMolecular Sequence DataPhylogenyRNA, BacterialRNA, Ribosomal, 16S

Summary

Culture-dependent and culture-independent characterization of Hirudo orientalis digestive tract reveals Aeromonas veronii and A. jandaei as cultured isolates plus uncultured Rikenella-like bacteria similar to those in H. verbana.

Why This Matters for Hirudotherapy

This study characterized the digestive-tract microbiota of the medicinal leech Hirudo orientalis using culture-dependent and culture-independent methods, culturing two Aeromonas species (A. veronii and A. jandaei) and identifying uncultured Rikenella-like bacteria most similar to isolates from H. verbana. The abstract notes that FDA-approved postoperative leech use can lead to bacterial infections, directly connecting the work to hirudotherapy safety. For ASH, this study is relevant because understanding the microbiota of medicinal leeches informs infection risk associated with leech therapy, particularly regarding Aeromonas species. The abstract is brief and provides limited detail on clinical implications; it does not report infection rates, clinical outcomes, or mitigation strategies, and characterization of microbiota composition appears to be the primary scope rather than therapeutic evaluation.

Citation

Characterization of the digestive-tract microbiota of Hirudo orientalis, a european medicinal leech.

Laufer AS, Siddall ME, Graf J · Applied and environmental microbiology, 2008

Added to ASH library: May 26, 2026 · Site last updated: June 18, 2026

This website provides educational information and does not constitute medical advice, diagnosis, or treatment recommendations. Medicinal leech therapy carries clinically meaningful risks and should be performed only by qualified clinicians under institutionally approved protocols. FDA 510(k) clearance for medicinal leeches is limited to specific indications; investigational and off-label discussions are labeled accordingly. For patient-specific guidance, consult a qualified healthcare provider.