American Society of Hirudotherapy

A new species of buffalo leech in the genusWhitman, 1886 (Arhynchobdellida, Hirudinidae) from Thailand.

Research article published in ZooKeys (2020)

Last Updated: June 18, 2026Reviewed by: ASH Editorial Board
Research article — evidence reviewArticle reference
Evidence: Research reportSalivary PharmacologyGenomics & ProteomicsJeratthitikul E et al. · ZooKeys, 2020

Abstract

Hirudinaria manillensis (Lesson, 1842), commonly known as the buffalo leech, shows a polymorphism of two ventral colorations. The green color morph has a plain green ventral surface and the red color morph has a brick-red ventral surface with two black submarginal stripes. Based on molecular and morphological evidence in the present study, these two color morphs were revealed as two different species. The red color morph fits well with the description of H. manillensis, while the green color morph showed some distinctions, and therefore is described herein as Hirudinaria thailandica Jeratthitikul & Panha, sp. nov. The new species can be distinguished from its congeners by the dark greenish or dark olive ventral surface and a round atrium with ventral insertion of ejaculatory ducts in the male reproductive organ. A phylogenetic tree based on concatenated data of COI and 28S genes supported the new species and further indicated it as a sister species to H. bpling Phillips, 2012.

Abstract sourced from PubMed (NCBI) for the cited record. See the original publication for the authoritative version.

Publication typeJournal Article

Summary

(Lesson, 1842), commonly known as the buffalo leech, shows a polymorphism of two ventral colorations. The green color morph has a plain green ventral surface and the red color morph has a brick-red ventral surface with two black submarginal stripes.

Why This Matters for Hirudotherapy

This taxonomic study used molecular (COI and 28S genes) and morphological evidence to show that two color morphs of the buffalo leech Hirudinaria manillensis are in fact two species, formally describing the green morph as a new species, Hirudinaria thailandica, sister to H. bpling. For ASH this is leech systematics and biodiversity background, not clinical or pharmacological evidence: the buffalo leech Hirudinaria differs from the medicinal leech Hirudo medicinalis used in hirudotherapy, and the abstract reports no salivary compounds, anticoagulant activity, or medical use. As a species-description and phylogenetics paper its relevance is limited to correctly identifying leech taxa, and no hirudotherapy conclusion should be inferred from it.

Citation

A new species of buffalo leech in the genusWhitman, 1886 (Arhynchobdellida, Hirudinidae) from Thailand.

Jeratthitikul E et al. · ZooKeys, 2020

Added to ASH library: May 28, 2026 · Site last updated: June 18, 2026

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