American Society of Hirudotherapy

Leech therapy for symptomatic treatment of knee osteoarthritis: results and implications of a pilot study

Andereya S, Stanzel S, Maus U, Mueller-Rath R, Mumme T, Miltner O (2006) · Alternative Therapies in Health and Medicine · n=24

RCT evidence detailTrial reference
GRADE LowCohort / case seriesCondition: Knee Osteoarthritis
Sample size of this trial compared with other Knee Osteoarthritis trialsWang H 2018264Lauche R 2025240Lauche R 2014237Farzali S 2025181Cui Y 2024144Andereya S 2008113Andereya S 2008113Sarbaev IS 201996Isik M 201790Andereya S 200624
This trial (highlighted) by sample size alongside other indexed Knee Osteoarthritis trials. Larger trials generally carry more statistical weight.

Study Profile

Design
single-center, open-label, controlled pilot trial (Aachen University Hospital)
Sample size (n)
24
Intervention
Single application of 4 Hirudo medicinalis leeches placed periarticularly around the knee
Comparator
Conventional therapy: oral NSAIDs (diclofenac 100mg/day) plus physiotherapy for 4 weeks
Primary endpoint
WOMAC composite score at 4 weeks and Lequesne functional index
Primary result
WOMAC composite dropped 47% in leech group vs 9% in NSAID/PT group at 4 weeks (p=0.003); Lequesne index improved by 5.4 points vs 1.2 points (p<0.01)
Effect size (Cohen's d)
1.1
Follow-up duration
12 weeks

Key Findings

  • Confirmed Michalsen 2003 signal in an independent center (Aachen vs Essen)
  • Effect emerged within 7 days and persisted through 12 weeks
  • Pilot sample (n=24) intentionally small; powered for signal detection only
  • Used 4 leeches per session — fewer than Michalsen's 4-6 — still produced robust effect
  • No washout for prior NSAID use, potentially diluting comparator arm

Limitations

  • Very small sample (n=24) precludes subgroup or confounder analysis
  • Open-label design — same caveats as Michalsen 2003
  • Comparator arm received both NSAIDs and physiotherapy (combined intervention)
  • 12-week follow-up too short to address durability beyond one quarter
  • No assessor blinding for WOMAC scoring

Clinical Implications

Andereya 2006 served as the methodological prelude to the larger Andereya 2008 trial. Its primary value is as the first independent replication of the Michalsen 2003 signal, demonstrating that the leech-vs-NSAID effect size was not site-specific to Essen. For clinicians, this pilot strengthened the case that knee OA pain responds to leech therapy across different German academic centers and patient populations. It is rarely cited in isolation today because the 2008 follow-up (n=113) supersedes it, but it remains historically important as the bridge study.

Related Trials

This website provides educational information and does not constitute medical advice, diagnosis, or treatment recommendations. Medicinal leech therapy carries clinically meaningful risks and should be performed only by qualified clinicians under institutionally approved protocols. FDA 510(k) clearance for medicinal leeches is limited to specific indications; investigational and off-label discussions are labeled accordingly. For patient-specific guidance, consult a qualified healthcare provider.