American Society of Hirudotherapy

Clinical Checklists

Standardized checklists for safe hirudotherapy practice

Last Updated: March 5, 2026Reviewed by: Andrei Dokukin, MD

Last updated: March 14, 2026

Standardized checklists reduce errors and ensure consistent adherence to clinical guidelines. These checklists are derived from published protocols (Mumcuoglu et al., 2014; Whitaker et al., 2004) and institutional best practices from academic medical centers that routinely perform leech therapy.

Pre-Procedure Checklist

Before First Leech Application

Patient Assessment:

  • Clinical indication documented
  • Contraindication screening completed
  • Allergy history reviewed (antibiotics, latex, prior leech reactions)
  • Medication review (anticoagulants, NSAIDs held per protocol)
  • Prior leech therapy history assessed

Documentation:

  • Signed informed consent in medical record
  • Off-label status disclosed (if applicable)
  • Baseline photographs taken
  • Leech source and lot number documented

Laboratory:

  • CBC with differential (Hgb ≥8 g/dL)
  • PT/INR (INR ≤3.0)
  • aPTT (≤2x upper normal)
  • Type and screen (surgical patients)
  • BMP (multi-day therapy patients)

Prophylaxis:

  • Antibiotic prophylaxis initiated (≥1 hour before)
  • Drug, dose, route documented
  • Allergy-appropriate regimen selected
  • Emergency supplies verified (epinephrine, hemostatics)
  • Biohazard waste container available

Intra-Procedure Checklist

During Leech Application

Setup:

  • Room temperature 72-77°F
  • "No Fragrances" notice posted
  • PPE donned (nitrile gloves, gown if splash risk)
  • Site cleansed with warm water only
  • Application sites marked
  • Absorbent pads positioned

Application:

  • Leech health verified (active, responsive)
  • Number of leeches per physician order
  • Attachment confirmed (peristaltic waves visible)
  • Patient NOT left unattended
  • Vital signs monitored per protocol
  • Feeding duration documented per leech

Post-Procedure Checklist

After Leech Detachment

Immediate:

  • Leech disposed in 70% ethyl alcohol
  • Death confirmed, transferred to biohazard container
  • Wound dressed (gauze, no pressure)
  • Post-detachment vital signs recorded
  • Bleeding assessment at 30 min and 2 hours
  • Session documented (# leeches, sites, duration, blood loss)

Discharge / Follow-Up:

  • Written wound care instructions provided
  • Return precautions reviewed (infection signs)
  • Antibiotic prophylaxis plan confirmed (continue duration)
  • Pain management instructions (acetaminophen; avoid NSAIDs)
  • Follow-up appointments scheduled (24h, 72h, 1wk, 4wk)
  • Contact information for emergencies provided

Serial Treatment Checklist

Multi-Day Surgical Leech Therapy

  • Cumulative blood loss tracked across all sessions
  • Hematocrit monitored every 4-8 hours
  • Blood products available (type and screen current)
  • Tissue viability assessment each session (color, Doppler, capillary refill)
  • Antibiotic prophylaxis continued throughout course
  • Post-course summary documented (total sessions, total leeches, total blood loss)
  • Follow-up schedule extends 26 days post-last application

Related Resources

This website provides educational information and does not constitute medical advice, diagnosis, or treatment recommendations. Medicinal leech therapy carries clinically meaningful risks and should be performed only by qualified clinicians under institutionally approved protocols. FDA 510(k) clearance for medicinal leeches is limited to specific indications; investigational and off-label discussions are labeled accordingly. For patient-specific guidance, consult a qualified healthcare provider.